Fixation is usually performed by incubation in a solution of a buffered chemical fixative, such as glutaraldehyde, sometimes in combination with formaldehyde[20][21][22] and other fixatives,[23] and optionally followed by postfixation with osmium tetroxide.[20] The fixed tissue is then dehydrated. Because air-drying causes collapse and shrinkage, this is commonly achieved by replacement of water in the cells with organic solvents such as ethanol or acetone, and replacement of these solvents in turn with a transitional fluid such as liquid carbon dioxide by critical point drying.[24] The carbon dioxide is finally removed while in a supercritical state, so that no gas–liquid interface is present within the sample during drying. https://www.youtube.com/watch?v=7bNPg8UbhaE&app=desktop

Submit website to directories (limited use). Professional search marketers don’t sub­mit the URL to the major search engines, but it’s possible to do so. A better and faster way is to get links back to your site naturally. Links get your site indexed by the search engines. However, you should submit your URL to directories such as Yahoo! (paid), Business.com (paid) and DMOZ (free). Some may choose to include AdSense (google.com/adsense) scripts on a new site to get their Google Media bot to visit. It will likely get your pages indexed quickly.
The impact on your results. It’s not a good idea to expect fast results from an SEO campaign, and it’s not fair to expect long-term results from a paid search campaign without investing enough in it. When employed correctly, SEO and paid search will fill in each other’s gaps and shore each other up with their strengths. SEO is ideal for branded keywords once a brand is established, whereas paid search is ideal for one-off advertising campaigns, such as a special deal with a specific expiration date. It pays to know which tactic you need for your digital marketing campaign.
"Organic" search engine marketing (organic search listings) combines the best practices of creativity, technology, usability, copy, and online promotion / PR. This is because many search engines base their relevancy algorithms on a combination of the text they see on a page, site, video, or image and combine the content information with external elements such as links and user behaviors/preferences demonstrated over time for a domain, content source, or specific content element.

In 2007, U.S. advertisers spent US $24.6 billion on search engine marketing.[3] In Q2 2015, Google (73.7%) and the Yahoo/Bing (26.3%) partnership accounted for almost 100% of U.S. search engine spend.[4] As of 2006, SEM was growing much faster than traditional advertising and even other channels of online marketing.[5] Managing search campaigns is either done directly with the SEM vendor or through an SEM tool provider. It may also be self-serve or through an advertising agency. As of October 2016, Google leads the global search engine market with a market share of 89.3%. Bing comes second with a market share of 4.36%, Yahoo comes third with a market share of 3.3%, and Chinese search engine Baidu is fourth globally with a share of about 0.68%.[6]
Next, consider your audience and the overall mood for your production. Are you targeting a small audience that will appreciate the newest, underground hip-hop track, or do you need something that will appeal to many demographics? Are you creating a practical product tutorial or an upbeat event recap? Be sure to choose music that enhances the overall tone of your video.
For example: If you sell household electronics and your paid ad promotes your selection of noise-cancelling headphones, your landing page should feature your noise-cancelling headphones. Directing people to your homepage instead, where they’ll need to sift through your product categories or do another search, will lead to frustration and a poor chance of getting the sale. http://www.youtube.com/embed/7bNPg8UbhaE
All sites have a home or "root" page, which is usually the most frequented page on the site and the starting place of navigation for many visitors. Unless your site has only a handful of pages, you should think about how visitors will go from a general page (your root page) to a page containing more specific content. Do you have enough pages around a specific topic area that it would make sense to create a page describing these related pages (for example, root page -> related topic listing -> specific topic)? Do you have hundreds of different products that need to be classified under multiple category and subcategory pages?
In 2007, Google announced a campaign against paid links that transfer PageRank.[29] On June 15, 2009, Google disclosed that they had taken measures to mitigate the effects of PageRank sculpting by use of the nofollow attribute on links. Matt Cutts, a well-known software engineer at Google, announced that Google Bot would no longer treat nofollowed links in the same way, to prevent SEO service providers from using nofollow for PageRank sculpting.[30] As a result of this change the usage of nofollow led to evaporation of PageRank. In order to avoid the above, SEO engineers developed alternative techniques that replace nofollowed tags with obfuscated Javascript and thus permit PageRank sculpting. Additionally several solutions have been suggested that include the usage of iframes, Flash and Javascript.[31] https://youtube.com/embed/7bNPg8UbhaE
The signals used by a scanning electron microscope to produce an image result from interactions of the electron beam with atoms at various depths within the sample. Various types of signals are produced including secondary electrons (SE), reflected or back-scattered electrons (BSE), characteristic X-rays and light (cathodoluminescence) (CL), absorbed current (specimen current) and transmitted electrons. Secondary electron detectors are standard equipment in all SEMs, but it is rare for a single machine to have detectors for all other possible signals.
The nature of the SEM's probe, energetic electrons, makes it uniquely suited to examining the optical and electronic properties of semiconductor materials. The high-energy electrons from the SEM beam will inject charge carriers into the semiconductor. Thus, beam electrons lose energy by promoting electrons from the valence band into the conduction band, leaving behind holes.
Search engines reward you when sites link to yours – they assume that your site must be valuable and you’ll rank higher in search results. And the higher the “rank” of the sites that link to you, the more they count in your own ranking. You want links from popular industry authorities, recognized directories, and reputable companies and organizations.
Paid inclusion is a search engine marketing method in itself, but also a tool of search engine optimization, since experts and firms can test out different approaches to improving ranking and see the results often within a couple of days, instead of waiting weeks or months. Knowledge gained this way can be used to optimize other web pages, without paying the search engine company.
Search queries—the words that users type into the search box—carry extraordinary value. Experience has shown that search engine traffic can make (or break) an organization's success. Targeted traffic to a website can provide publicity, revenue, and exposure like no other channel of marketing. Investing in SEO can have an exceptional rate of return compared to other types of marketing and promotion.
Google's core algorithms and its propensity to shroud its data in layers of obscurity is not something new. However, it is critical to any understanding of marketing on the internet simply because this visibility is at the heart of everything else that you do. Forget about social media and other forms of marketing for the time being. Search engine optimization (SEO) offers up the proverbial key to near-limitless amounts of traffic on the web.

Consider how well you know your industry. If you have been in business for a while and already know what your customers want and how to best reach them, you may want to start to build a long-term SEO strategy that will provide value over time. If you aren’t sure how customers and competitors will respond to your offerings or content, you may want to consider an SEM campaign that allows you to test your ideas, products, and services. Use these sites for market research to better understand your target audience and your position in the industry. https://www.youtube.com/v/7bNPg8UbhaE&feature=youtu.be
Magnification in an SEM can be controlled over a range of about 6 orders of magnitude from about 10 to 500,000 times. Unlike optical and transmission electron microscopes, image magnification in an SEM is not a function of the power of the objective lens. SEMs may have condenser and objective lenses, but their function is to focus the beam to a spot, and not to image the specimen. Provided the electron gun can generate a beam with sufficiently small diameter, an SEM could in principle work entirely without condenser or objective lenses, although it might not be very versatile or achieve very high resolution. In an SEM, as in scanning probe microscopy, magnification results from the ratio of the dimensions of the raster on the specimen and the raster on the display device. Assuming that the display screen has a fixed size, higher magnification results from reducing the size of the raster on the specimen, and vice versa. Magnification is therefore controlled by the current supplied to the x, y scanning coils, or the voltage supplied to the x, y deflector plates, and not by objective lens power.
Search Engine Optimization (SEO): Maximizes search result exposure by targeting and driving Web traffic and ultimately delivering increased sales. The majority of search engine page results come from unpaid or organic search. SEO optimizes websites to ensure top search engine placement, such as Google, as only 15 percent of searchers continue beyond page one.
Collaborative Environment: A collaborative environment can be set up between the organization, the technology service provider, and the digital agencies to optimize effort, resource sharing, reusability and communications.[36] Additionally, organizations are inviting their customers to help them better understand how to service them. This source of data is called User Generated Content. Much of this is acquired via company websites where the organization invites people to share ideas that are then evaluated by other users of the site. The most popular ideas are evaluated and implemented in some form. Using this method of acquiring data and developing new products can foster the organizations relationship with their customer as well as spawn ideas that would otherwise be overlooked. UGC is low-cost advertising as it is directly from the consumers and can save advertising costs for the organisation.

The leading search engines, such as Google, Bing and Yahoo!, use crawlers to find pages for their algorithmic search results. Pages that are linked from other search engine indexed pages do not need to be submitted because they are found automatically. The Yahoo! Directory and DMOZ, two major directories which closed in 2014 and 2017 respectively, both required manual submission and human editorial review.[39] Google offers Google Search Console, for which an XML Sitemap feed can be created and submitted for free to ensure that all pages are found, especially pages that are not discoverable by automatically following links[40] in addition to their URL submission console.[41] Yahoo! formerly operated a paid submission service that guaranteed crawling for a cost per click;[42] however, this practice was discontinued in 2009.


However, some of the world's top-earning blogs gross millions of dollars per month on autopilot. It's a great source of passive income and if you know what you're doing, you could earn a substantial living from it. You don't need millions of visitors per month to rake in the cash, but you do need to connect with your audience and have clarity in your voice. http://m.youtube.com/watch?feature=player_embedded&v=e29F5n3ea0I
The first commercial development of the ESEM in the late 1980s[32][33] allowed samples to be observed in low-pressure gaseous environments (e.g. 1–50 Torr or 0.1–6.7 kPa) and high relative humidity (up to 100%). This was made possible by the development of a secondary-electron detector[34][35] capable of operating in the presence of water vapour and by the use of pressure-limiting apertures with differential pumping in the path of the electron beam to separate the vacuum region (around the gun and lenses) from the sample chamber.
What is Search Engine Optimization (also known as SEO)? A broad definition is that search engine optimization is the art and science of making web pages attractive to search engines. More narrowly, SEO seeks to tweak particular factors known to affect search engine standing to make certain pages more attractive to search engines than other web pages that are vying for the same keywords or keyword phrases.
Did you know that 85% of Facebook video is watched with no sound? Many people watch videos in places where they can't turn the sound up, or where there's background noise. Those are two important reasons why video captions matter. And, of course, video captions matter for accessibility. Include captions, and you will improve you click-through rate as those with hearing loss or impairment can still understand your video content. Captions also help with SEO by providing text that search engines can find.

AdWords is recognized as a web-based advertising utensil since it adopts keywords which can deliver adverts explicitly to web users looking for information in respect to a certain product or service. It is flexible and provides customizable options like Ad Extensions, access to non-search sites, leveraging the display network to help increase brand awareness. The project hinges on cost per click (CPC) pricing where the maximum cost per day for the campaign can be chosen, thus the payment of the service only applies if the advert has been clicked. SEM companies have embarked on AdWords projects as a way to publicize their SEM and SEO services. One of the most successful approaches to the strategy of this project was to focus on making sure that PPC advertising funds were prudently invested. Moreover, SEM companies have described AdWords as a practical tool for increasing a consumer’s investment earnings on Internet advertising. The use of conversion tracking and Google Analytics tools was deemed to be practical for presenting to clients the performance of their canvas from click to conversion. AdWords project has enabled SEM companies to train their clients on the utensil and delivers better performance to the canvass. The assistance of AdWord canvass could contribute to the growth of web traffic for a number of its consumer’s websites, by as much as 250% in only nine months.[30]


Maintenance. Ongoing addition and modification of keywords and website con­tent are necessary to continually improve search engine rankings so growth doesn’t stall or decline from neglect. You also want to review your link strategy and ensure that your inbound and outbound links are relevant to your business. A blog can provide you the necessary structure and ease of content addition that you need. Your hosting company can typically help you with the setup/installation of a blog.
I liken this to a paradoxical Catch-22 scenario, because it seems like without one you can't have the other. It takes money to drive traffic, but it takes traffic to make money. So don't make the mistake that millions of other online marketers make around the world. Before you attempt to scale or send any semblance of traffic to your offers, be sure to split-test things to oblivion and determine your conversion rates before diving in headfirst.

Was reviewing some competitive data and thought this was pretty interesting. I ran a batch analysis on Ahrefs of competitors. See attached screenshot. With just 603 backlinks, Our site is ranking up there with sites with 2x, 3x, 10x the number of backlinks/unique ips. Guessing some of this authority is coming from the backlinks program and general good quality of those links. Hard to speculate but nice to see. Ben R. http://www.youtube.com/e/7bNPg8UbhaE
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