Fixation is usually performed by incubation in a solution of a buffered chemical fixative, such as glutaraldehyde, sometimes in combination with formaldehyde[20][21][22] and other fixatives,[23] and optionally followed by postfixation with osmium tetroxide.[20] The fixed tissue is then dehydrated. Because air-drying causes collapse and shrinkage, this is commonly achieved by replacement of water in the cells with organic solvents such as ethanol or acetone, and replacement of these solvents in turn with a transitional fluid such as liquid carbon dioxide by critical point drying.[24] The carbon dioxide is finally removed while in a supercritical state, so that no gas–liquid interface is present within the sample during drying. https://www.youtube.com/watch?v=7bNPg8UbhaE&app=desktop
A note about shooting with two cameras: Your editor will need to sync the footage between the different views. To help them do this, clap your hands loudly in the view of both cameras right before you ask the first interview question … yes, just like an old fashion clapboard. Modern editing software has auto-sync features, but this loud clap will help you initially line up the clips.
By using Internet platforms, businesses can create competitive advantage through various means. To reach the maximum potential of digital marketing, firms use social media as its main tool to create a channel of information. Through this a business can create a system in which they are able to pinpoint behavioral patterns of clients and feedback on their needs.[30] This means of content has shown to have a larger impingement on those who have a long-standing relationship with the firm and with consumers who are relatively active social media users. Relative to this, creating a social media page will further increase relation quality between new consumers and existing consumers as well as consistent brand reinforcement therefore improving brand awareness resulting in a possible rise for consumers up the Brand Awareness Pyramid.[31] Although there may be inconstancy with product images;[32] maintaining a successful social media presence requires a business to be consistent in interactions through creating a two way feed of information; firms consider their content based on the feedback received through this channel, this is a result of the environment being dynamic due to the global nature of the internet.[29] Effective use of digital marketing can result in relatively lowered costs in relation to traditional means of marketing; Lowered external service costs, advertising costs, promotion costs, processing costs, interface design costs and control costs.[32]
Optimization techniques are highly tuned to the dominant search engines in the target market. The search engines' market shares vary from market to market, as does competition. In 2003, Danny Sullivan stated that Google represented about 75% of all searches.[62] In markets outside the United States, Google's share is often larger, and Google remains the dominant search engine worldwide as of 2007.[63] As of 2006, Google had an 85–90% market share in Germany.[64] While there were hundreds of SEO firms in the US at that time, there were only about five in Germany.[64] As of June 2008, the marketshare of Google in the UK was close to 90% according to Hitwise.[65] That market share is achieved in a number of countries.

Thanks for referencing some of work here Liis. Like everything online, though, you need to be strategic in promoting your video. The content, messaging, and the promotion channels all contribute to the success or failure of your video marketing strategy.We wrote an interesting article here based on a related subjec, hope you like it ! https://thevideoanimationcompany.com/marketing/what-is-an-explainer-video-and-do-you-really-need-one

Black hat SEO attempts to improve rankings in ways that are disapproved of by the search engines, or involve deception. One black hat technique uses text that is hidden, either as text colored similar to the background, in an invisible div, or positioned off screen. Another method gives a different page depending on whether the page is being requested by a human visitor or a search engine, a technique known as cloaking. Another category sometimes used is grey hat SEO. This is in between black hat and white hat approaches, where the methods employed avoid the site being penalized, but do not act in producing the best content for users. Grey hat SEO is entirely focused on improving search engine rankings.
To make your videos as memorable as possible, ensure that you’re keeping them in line with your brand strategy. This means keeping colors, fonts, logos and voice the same in your video marketing as they are in your blogs and articles. While videos do things text content doesn’t, users should still be able to recognize the style and format of your brand’s videos online. https://www.youtube.com/v/e29F5n3ea0I&feature=youtu.be
SEM samples are prepared to withstand the vacuum conditions and the high energy beam of electrons, and have to be small enough to fit on the specimen stage. Samples are generally mounted rigidly to a specimen holder or stub using a conductive adhesive. SEM is used extensively for defect analysis of semiconductor wafers, and manufacturers make instruments that can examine any part of a 300 mm semiconductor wafer. Many instruments have chambers that can tilt an object of that size to 45° and provide continuous 360° rotation.
The next step? How will you communicate with people. Sharpe says that you need to decide on this early on. Will you blog? Will you use social media? Will you build a list by working with solo ad providers? Will you place paid advertisements? What will you do and how will you do it? What you must realize here is that you have to get really good at copy writing. The better you get at copy writing, the more success you'll find as an internet marketer.
Multi-Resolution reconstruction using single 2D File: High-quality 3D imaging may be an ultimate solution for revealing the complexities of any porous media, but acquiring them is costly and time consuming. High-quality 2D SEM images, on the other hand, are widely available. Recently, a novel three-step, multiscale, multiresolution reconstruction method is presented that directly uses 2D images in order to develop 3D models. This method, based on a Shannon Entropy and conditional simulation, can be used for most of the available stationary materials and can build various stochastic 3D models just using a few thin sections.[56]
However, some of the world's top-earning blogs gross millions of dollars per month on autopilot. It's a great source of passive income and if you know what you're doing, you could earn a substantial living from it. You don't need millions of visitors per month to rake in the cash, but you do need to connect with your audience and have clarity in your voice. https://www.youtube.com/v/7bNPg8UbhaE
There’s no avoiding it: internet marketing is critical for the success of your business in 2018. But with all the gimmicks and tricks, it can be difficult to distinguish short-term wins from effective long-term strategies, which is why we’ve created an ultimate guide. Here, we’ll cover everything from marketing strategies to real-world examples, to ensure your business reaches the right people out of that four billion. http://youtube.com/e/e29F5n3ea0I?app=desktop
The truth? Today, rising above the noise and achieving any semblance of visibility has become a monumental undertaking. While we might prevail at searching, we fail at being found. How are we supposed to get notice while swimming in a sea of misinformation and disinformation? We've become immersed in this guru gauntlet where one expert after another is attempting to teach us how we can get the proverbial word out about our businesses and achieve visibility to drive more leads and sales, but we all still seem to be lost.
This method requires an SEM image obtained in oblique low angle lighting. The grey-level is then interpreted as the slope, and the slope integrated to restore the specimen topography. This method is interesting for visual enhancement and the detection of the shape and position of objects ; however the vertical heights cannot usually be calibrated, contrary to other methods such as photogrammetry.[41] https://www.youtube.com/watch?v=e29F5n3ea0I&app=mobile
Online marketing is the practice of leveraging web-based channels to spread a message about a company’s brand, products, or services to its potential customers. The methods and techniques used for online marketing include email, social media, display advertising, search engine optimization, and more. The objective of marketing is to reach potential customers through the channels where they spend time reading, searching, shopping, or socializing online.
Internet Live Stats claims that there are over 66,000 searches per second on Google each day. (Note that this is figure was taken at the time of writing – if you click on the link you’ll likely find a different number, as data from this source is collected in real time.) That’s a lot of activity for any given business to compete with and is why SEO is such an important (but tricky) area of digital marketing to know. 
Place strategic search phrases on pages. Integrate selected keywords into your website source code and existing content on designated pages. Make sure to apply a sug­gested guideline of one to three keywords/phrases per content page and add more pages to complete the list. Ensure that related words are used as a natural inclu­sion of your keywords. It helps the search engines quickly determine what the page is about. A natural approach to this works best. In the past, 100 to 300 words on a page was recommended. Many tests show that pages with 800 to 2,000 words can outperform shorter ones. In the end, the users, the marketplace, content and links will determine the popularity and ranking numbers. http://www.youtube.com/v/7bNPg8UbhaE&feature=youtu.be
From these challenges, rise video marketing best practices. And it all comes down to strategy: If you want your video marketing to have results, then you must plan, test, analyze, and test again. Yes, the goal of your videos is to tell a story and engage your customers but, from a marketing perspective, your videos must also align to your sales funnel. What is the purpose of each video? Who is the audience? How does the video further your marketing goals? https://youtube.com/e/7bNPg8UbhaE?app=desktop
While the obvious purpose of internet marketing is to sell goods, services or advertising over the internet, it's not the only purpose a business using internet marketing may have; a company may be marketing online to communicate a message about itself (building its brand) or to conduct research. Online marketing can be a very effective way to identify a target market or discover a marketing segment's wants and needs. (Learn more about conducting market research).
Online reviews, then, have become another form of internet marketing that small businesses can't afford to ignore. While many small businesses think that they can't do anything about online reviews, that's not true. Just by actively encouraging customers to post reviews about their experience small businesses can weight online reviews positively. Sixty-eight percent of consumers left a local business review when asked. So assuming a business's products or services are not subpar, unfair negative reviews will get buried by reviews by happier customers.

Google’s Accelerated Mobile Project (AMP) will gain even more popularity. AMP is a collaboration between developers and other industry professionals to create a massive open source library that offers users more opportunity to create quick and smooth mobile-friendly web pages. This is one area where we’ll be seeing the death of slow-loading pages that rank low. The use of AMP has resulted in huge increases in mobile searches and traffic from major publications such as the Washington Post and Slate.


As of October 2018 almost 4.2 billion people were active internet users and 3.4 billion were social media users (Statista). China, India and the United States rank ahead all other countries in terms of internet users. This gives a marketer an unprecedented number of customers to reach with product and service offerings, available 24 hours a day, seven days a week. The interactive nature of the internet facilitates immediate communication between businesses and consumers, allowing businesses to respond quickly to the needs of consumers and changes in the marketplace.
Another one of the benefits of SEM is that people who see your PPC ads are those most likely to want to buy your product or service. PPC ads require you to choose a geographic location and specific search queries to target. As a result, you can be sure that anyone who clicks on your ad is not arbitrarily surfing the web, but rather, is looking for your product or service and in a position to do so.
Facebook Ads and other social media ad platforms, for example, are pay-per-click platforms that do not fall under the SEM category. Instead of showing your ads to people who are searching for similar content like search ads do, social media sites introduce your product to people who happen to be just browsing through their feeds. These are two very, very different types of online advertising.
Influencer marketing: Important nodes are identified within related communities, known as influencers. This is becoming an important concept in digital targeting. It is possible to reach influencers via paid advertising, such as Facebook Advertising or Google Adwords campaigns, or through sophisticated sCRM (social customer relationship management) software, such as SAP C4C, Microsoft Dynamics, Sage CRM and Salesforce CRM. Many universities now focus, at Masters level, on engagement strategies for influencers. http://youtube.com/v/7bNPg8UbhaE?version=3
The leading search engines, such as Google, Bing and Yahoo!, use crawlers to find pages for their algorithmic search results. Pages that are linked from other search engine indexed pages do not need to be submitted because they are found automatically. The Yahoo! Directory and DMOZ, two major directories which closed in 2014 and 2017 respectively, both required manual submission and human editorial review.[39] Google offers Google Search Console, for which an XML Sitemap feed can be created and submitted for free to ensure that all pages are found, especially pages that are not discoverable by automatically following links[40] in addition to their URL submission console.[41] Yahoo! formerly operated a paid submission service that guaranteed crawling for a cost per click;[42] however, this practice was discontinued in 2009.

We still seem to have PageRank as an important ranking factor from Google, but have been told that Google is using a machine learning approach called RankBrain. The focus of this RankBrain approach is to help the search engine understand the meaning of queries better, and provide answers that (still) focus upon meeting the situational and informational needs of searchers.
You should optimize your site to serve your users' needs. One of those users is a search engine, which helps other users discover your content. Search Engine Optimization is about helping search engines understand and present content. Your site may be smaller or larger than our example site and offer vastly different content, but the optimization topics we discuss below should apply to sites of all sizes and types. We hope our guide gives you some fresh ideas on how to improve your website, and we'd love to hear your questions, feedback, and success stories in the Google Webmaster Help Forum1. https://www.youtube.com/watch?feature=youtu.be&v=e29F5n3ea0I
The field is replete with terms that might confuse and perplex the average individual. What is a squeeze page? What's a sales funnel? What's a CPA? What's SEO? How do you setup a good blog to filter the right type of relevant traffic and get your offer in front of eligible users? What's a massive value post (MVP) really mean? Clearly, there are an endless array of terms, some of which you might already know or might not depending on how much you presently know about the field.

Hey Liis, Great post with a lot is stats that speak the modern scenario. Video marketing can be leveraged even better when it is a startup company. Given that startups have a limited budget and it is also very necessary for them to be noticed, animated videos are a great option. I recently wrote a blog post on why every startup company should use video marketing http://www.pixelgenio.com/video-marketing/6-reasons-every-startup-company-use-video-marketing I would appreciate your comments on my writing. Cheers!
Search Engine Marketing (SEM) is a descriptor that incorporates various types of paid search advertising. This is the type of thing that you’ll see as Google ad and usually ends up on the top of a page list. Usually, this has a to do with keyword placement and usage and that’s one way why doing it well can require some extra knowledge and strategy.

The most common imaging mode collects low-energy (<50 eV) secondary electrons that are ejected from the k-shell of the specimen atoms by inelastic scattering interactions with beam electrons. Due to their low energy, these electrons originate within a few nanometers from the sample surface.[14] The electrons are detected by an Everhart-Thornley detector,[29] which is a type of scintillator-photomultiplier system. The secondary electrons are first collected by attracting them towards an electrically biased grid at about +400 V, and then further accelerated towards a phosphor or scintillator positively biased to about +2,000 V. The accelerated secondary electrons are now sufficiently energetic to cause the scintillator to emit flashes of light (cathodoluminescence), which are conducted to a photomultiplier outside the SEM column via a light pipe and a window in the wall of the specimen chamber. The amplified electrical signal output by the photomultiplier is displayed as a two-dimensional intensity distribution that can be viewed and photographed on an analogue video display, or subjected to analog-to-digital conversion and displayed and saved as a digital image. This process relies on a raster-scanned primary beam. The brightness of the signal depends on the number of secondary electrons reaching the detector. If the beam enters the sample perpendicular to the surface, then the activated region is uniform about the axis of the beam and a certain number of electrons "escape" from within the sample. As the angle of incidence increases, the interaction volume increases and the "escape" distance of one side of the beam decreases, resulting in more secondary electrons being emitted from the sample. Thus steep surfaces and edges tend to be brighter than flat surfaces, which results in images with a well-defined, three-dimensional appearance. Using the signal of secondary electrons image resolution less than 0.5 nm is possible. http://youtu.be/e29F5n3ea0I
Using Dr Dave Chaffey's approach, the digital marketing planning (DMP) has three main stages: Opportunity, Strategy and Action. He suggests that any business looking to implement a successful digital marketing strategy must structure their plan by looking at opportunity, strategy and action. This generic strategic approach often has phases of situation review, goal setting, strategy formulation, resource allocation and monitoring.[59]
Sixty-five percent of business decision-makers visit a marketer’s website after viewing a branded video. It’s clear that quality and relevant video marketing content can dramatically improve your site’s SEO by driving people to your homepage. Additionally, video can enhance your conversion rates: HubSpot reports that 39% of business decision-makers contact a vendor after viewing a branded video.
Entertainment — The Internet is a plethora of things to do. Some are looking for videos. Others are looking for games. Many are entertained by new stories. Others like financial calculators. Others want to learn something new. Think about what kind of entertainment your target is looking for. Relate it back to your brand. That’s a great way to use SEM to connect.

Email marketing is the practice of nurturing leads and driving sales through email communications with your customers. Like social media, the goal is to remind users that you’re here and your product is waiting. Unlike social media, however, you can be a lot more aggressive with your sales techniques, as people expect that email marketing will contain offers, product announcements and calls to action.


Prioritizing clicks refers to display click ads, although advantageous by being ‘simple, fast and inexpensive’ rates for display ads in 2016 is only 0.10 percent in the United States. This means one in a thousand click ads are relevant therefore having little effect. This displays that marketing companies should not just use click ads to evaluate the effectiveness of display advertisements (Whiteside, 2016).[42] https://www.youtube.com/watch?feature=player_embedded&v=e29F5n3ea0I
Sharpe says that you shouldn't dive into internet marketing until you decide on a niche and figure out what you're passionate about. Do you want to join the make-money-online (MMO) niche? Or do you want to engage in another niche? For example, you could sell products or online courses about blogging or search engine optimization or anything else for that matter. Keep in mind that whatever you're selling, whatever niche you're in, that you need to embed yourself there deeply.
Our SEO professionals are all well-respected thought leaders in the space and have decades of combined experience and include the following credentials: Search Engine Workshop Certification, Google Analytics and Yahoo Certifications, PMP Certification, UNIX Certification, Computer Engineering degrees and MBA’s. Our SEO team members are acclaimed SEO speakers and bloggers. IMI’s SEO team members have been keynote presenters at Pubcon, SMX, SEMCon, Etail, and many more influential conferences.
When they’re considering buying something and want to research their options, there’s a variety of methods used. Some of the top tactics are a search engine, visiting a company or product’s official website, looking at review websites, and watching videos. Video could easily be incorporated into every one of those places, giving your brand a better chance of convincing the consumer.

A content specialist needs to be a Jack or Jill of all trades, utilizing excellent written and verbal communication skills, above-average computer literacy, and a natural interest in trends. This job is ultimately about translating the key aspects of the product into content the target demographic finds appealing. This is part art, part critical thinking, and 100% attention to detail.
In the beginning, it was rough for Sharpe. No one out there should think that it's going to be easy whatsoever. His journey took years and years to go from an absolute beginner, to a fluid and seasoned professional, able to clearly visualize and achieve his dreams, conveying his vast knowledge expertly to those hungry-minded individuals out there looking to learn how to generate a respectable income online.

Search engine optimization consultants expanded their offerings to help businesses learn about and use the advertising opportunities offered by search engines, and new agencies focusing primarily upon marketing and advertising through search engines emerged. The term "search engine marketing" was popularized by Danny Sullivan in 2001[12] to cover the spectrum of activities involved in performing SEO, managing paid listings at the search engines, submitting sites to directories, and developing online marketing strategies for businesses, organizations, and individuals.
The scanning electron microscope (SEM) uses a focused beam of high-energy electrons to generate a variety of signals at the surface of solid specimens. The signals that derive from electron-sample interactions reveal information about the sample including external morphology (texture), chemical composition, and crystalline structure and orientation of materials making up the sample. In most applications, data are collected over a selected area of the surface of the sample, and a 2-dimensional image is generated that displays spatial variations in these properties. Areas ranging from approximately 1 cm to 5 microns in width can be imaged in a scanning mode using conventional SEM techniques (magnification ranging from 20X to approximately 30,000X, spatial resolution of 50 to 100 nm). The SEM is also capable of performing analyses of selected point locations on the sample; this approach is especially useful in qualitatively or semi-quantitatively determining chemical compositions (using EDS), crystalline structure, and crystal orientations (using EBSD). The design and function of the SEM is very similar to the EPMA and considerable overlap in capabilities exists between the two instruments.
Not every single ad will appear on every single search. This is because the ad auction takes a variety of factors into account when determining the placement of ads on the SERP, and because not every keyword has sufficient commercial intent to justify displaying ads next to results. However, the two main factors that Google evaluates as part of the ad auction process are your maximum bid and the Quality Score of your ads.
Video can also provide social proof for your product or service. According to WebDam, customer testimonials have the highest effectiveness rating for content marketing at 89%. When creating video success stories focus on the story of your customer and the success he/she achieved from using your product/service. This nifty question template from Copyblogger will help you create awesome and human-focused video and written testimonials.
Google recommends that all websites use https:// when possible. The hostname is where your website is hosted, commonly using the same domain name that you'd use for email. Google differentiates between the "www" and "non-www" version (for example, "www.example.com" or just "example.com"). When adding your website to Search Console, we recommend adding both http:// and https:// versions, as well as the "www" and "non-www" versions.
Some marketers believe that there are "tricks" that will improve the relevancy of sites within the search engines that are spider- (crawler-) based. Not only do some of these tricks not work; many of them can result in negative relevance penalties as the engines take measures to punish search marketers seeking to manipulate ranking and relevance. That said, there are still compelling reasons to put legitimate efforts behind organic SEO optimization, particularly efforts in site design, content formatting, content clarity optimization, and server platform adjustments.
Magnification in an SEM can be controlled over a range of about 6 orders of magnitude from about 10 to 500,000 times. Unlike optical and transmission electron microscopes, image magnification in an SEM is not a function of the power of the objective lens. SEMs may have condenser and objective lenses, but their function is to focus the beam to a spot, and not to image the specimen. Provided the electron gun can generate a beam with sufficiently small diameter, an SEM could in principle work entirely without condenser or objective lenses, although it might not be very versatile or achieve very high resolution. In an SEM, as in scanning probe microscopy, magnification results from the ratio of the dimensions of the raster on the specimen and the raster on the display device. Assuming that the display screen has a fixed size, higher magnification results from reducing the size of the raster on the specimen, and vice versa. Magnification is therefore controlled by the current supplied to the x, y scanning coils, or the voltage supplied to the x, y deflector plates, and not by objective lens power.
After you’ve determined the type of music you need, it’s time to start analyzing potential songs. Consider the song’s pacing. Songs with a steady rhythm are easy to change to suit your video style. Hoping to include your favorite, Top 40 hit? Popular, radio songs are usually structured in 4-5 parts and can be difficult to transition. Try to choose simple songs that are easy to loop. If you’re looking for an instrumental song, be sure to find something that was recorded with real instruments. Songs made with digital samples can make your video feel unprofessional and out of date.

Consider the age of your business. If you just opened your business and launched your website, it’s going to take time to develop your SEO and begin to appear organically in the search. While that doesn’t mean you shouldn’t put together an SEO strategy, it does mean that you could benefit from an SEM strategy until you build your SEO. SEM is an effective way to drive traffic while building organic SEO. http://y2u.be/7bNPg8UbhaE

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